Superficial papillary urothelial neoplasms of the bladder (PTA E PT1): correlation of expression of P53, KI-67 and CK20 with histologic grade, recurrence and tumor progression.

OBJECTIVE: To investigate the immunohistochemical expressions of p53, ki67, CK20 in superficial papillary urothelial neoplasms of the bladder and correlate them with histological grade, tumor progression and recurrence. METHODS: We selected samples of 43 patients with superficial transitional cell carcinoma of the bladder. They were divided into two groups, one called Recurrent (R), with 18 individuals, and other Non-Recurrent (NR), with 25. Multi-sampling blocks were prepared. The immunohistochemical technique employed was immunoperoxidase, and the antibodies were: p53: Novocastra (clone DO7) at a dilution of 1/100; Ki67: Spring (clone SP6) at a dilution of 1/100; and CK20: Dako (clone K20 .8) at a dilution of 1/50. RESULTS: The expression of p53 was observed in 11 cases, six in the Recurrent group and five in the Non-Recurrent, all high-grade tumors (p = 0.0001). The histological progression occurred in six patients (p = 0.0076). Of the 18 Recurrent cases, six showed immunoreactivity for p53 and 12 were negative for this antibody (p = 0.1715). Ki67 was positive in 17 of the 18 cases from the Recurrent group (p = 0.0001) and, from 20 high-grade tumors, 18 showed reaction to this antibody (p = 0.0001). Of the 18 individuals who had recurrence, 13 showed anomalous expression for CK20 (p = 0.0166). In high-grade carcinomas, of the 20 cases, 16 showed anomalous expression for this antibody, while 18 of the 23 patients with low-grade tumors showed normal expression for CK20 (p = 0.0002). CONCLUSION: The p53 showed good correlation with histological progression and histologic grade. Ki67 was strongly associated with recurrence and histological grade, and CK20 was also associated with these variables.

Neoplasias uroteliais papilíferas superficiais da bexiga (pTa e pT1): correlação da expressão do p53, KI-67 E CK20 com grau histológico, recidiva e progressão tumoral / Superficial papillary urothelial neoplasms of the bladder (PTA E PT1): correlation of expression of P53, KI-67 and CK20 with histologic grade, recurrence and tumor progression

OBJETIVO: Investigar a expressão imunoistoquímica dos marcadores p53, Ki-67, CK20 em neoplasias uroteliais papilíferas superficiais da bexiga e correlacionar com o grau histológico, progressão tumoral e recidiva. MÉTODOS: Foram selecionadas amostras de 43 pacientes portadores de carcinoma de células transicionais superficiais da bexiga. Elas foram distribuídas em dois grupos, um denominado recorrente, de 18 indivíduos e outro não recorrente, com 25 casos. Foram confeccionados blocos multiamostrais. A técnica imunoistoquímica empregada foi de imunoperoxidase e os anticorpos foram: p53 (clone DO7), o Ki-67 (clone SP6) e CK20. RESULTADOS: A expressão do p53 foi observada em 11 casos, todos tumores de alto grau (p=0,0001). A progressão histológica ocorreu em seis indivíduos (p=0,0076). Dos 18 casos recorrentes, seis apresentaram imunorreação para o p53 e 12 foram negativos para este anticorpo (p=0,1715). O Ki-67 foi positivo em 17 dos 18 casos do grupo recorrente (p=0,0001) e dos 20 tumores de alto grau, 18 apresentaram reação para este anticorpo (p=0,0001). Dos 18 indivíduos que tiveram recorrência, 13 apresentaram expressão anômala para CK20 (p=0,0166). Nos carcinomas de alto grau, dos 20 casos, 16 apresentaram expressão anômala para este anticorpo, enquanto que 18 dos 23 indivíduos com tumores de baixo grau mostraram expressão habitual para a CK20 (p=0,0002). CONCLUSÃO: O p53 mostrou boa correlação com a progressão histológica e grau histológico. O Ki-67 apresentou forte associação com a recidiva e grau histológico, e a CK20 também associou-se com estas variáveis.

OBJECTIVE: To investigate the immunohistochemical expressions of p53, ki67, CK20 in superficial papillary urothelial neoplasms of the bladder and correlate them with histological grade, tumor progression and recurrence. METHODS: We selected samples of 43 patients with superficial transitional cell carcinoma of the bladder. They were divided into two groups, one called Recurrent (R), with 18 individuals, and other Non-Recurrent (NR), with 25. Multi-sampling blocks were prepared. The immunohistochemical technique employed was immunoperoxidase, and the antibodies were: p53: Novocastra (clone DO7) at a dilution of 1/100; Ki67: Spring (clone SP6) at a dilution of 1/100; and CK20: Dako (clone K20 .8) at a dilution of 1/50. RESULTS: The expression of p53 was observed in 11 cases, six in the Recurrent group and five in the Non-Recurrent, all high-grade tumors (p = 0.0001). The histological progression occurred in six patients (p = 0.0076). Of the 18 Recurrent cases, six showed immunoreactivity for p53 and 12 were negative for this antibody (p = 0.1715). Ki67 was positive in 17 of the 18 cases from the Recurrent group (p = 0.0001) and, from 20 high-grade tumors, 18 showed reaction to this antibody (p = 0.0001). Of the 18 individuals who had recurrence, 13 showed anomalous expression for CK20 (p = 0.0166). In high-grade carcinomas, of the 20 cases, 16 showed anomalous expression for this antibody, while 18 of the 23 patients with low-grade tumors showed normal expression for CK20 (p = 0.0002). CONCLUSION: The p53 showed good correlation with histological progression and histologic grade. Ki67 was strongly associated with recurrence and histological grade, and CK20 was also associated with these variables.